A reagentless signal-on architecture for electronic, aptamer-based sensors via target-induced strand displacement.
نویسندگان
چکیده
Thrombin binding stabilizes the alternative G-quadruplex conformation of the aptamer, liberating the methylene blue (MB)-tagged oligonucleotide to produce a flexible, single-stranded DNA element. This allows the MB tag to collide with the gold electrode surface, producing a readily detectable Faradaic current at thrombin concentrations as low as approximately 3 nM.
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ورودعنوان ژورنال:
- Journal of the American Chemical Society
دوره 127 51 شماره
صفحات -
تاریخ انتشار 2005